Group N Streptococcal Phage Lysin

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Streptococcal Phage - Associated Lysin

I t has been reported by Maxted (1) and Krause (2) that lysates of Group C streptococci infected with C1 bacteriophage contained an enzyme that had the ability to lyse Groups A, C, and E streptococci and their isolated cell walls. Crude and partially purified preparations of this enzyme were found to be useful for the isolation of Group A streptococcal cell wall components (M protein and C carb...

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Purification and Physical Properties of Group C Streptococcal Phage-associated Lysin

A purification procedure for the Group C phage-associated lysin is described utilizing tetrathionate to protect the enzyme's -SH group(s) from thiol-inactivating agents. A 652-fold purification has been accomplished yielding a solution in which the enzyme activity corresponds to essentially a single band on polyacrylamide gel which accounts for 70% of the total protein in the preparation. A mol...

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Preparation and Antigenicity of M Protein Released from Group A, Type 1 Streptococcal Cell Walls by Phage-associated Lysin

The lysis of Group A type 1 streptococcal cell walls by phage-associated lysin has been described. In the preparation of lysin, a new semisynthetic non-protein media to support growth of the propagating strain of Group C streptococci was employed. Following lysis of the cell walls, the resulting digest was partially purified by ion-exchange chromatography and ammonium sulfate precipitation. In ...

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Dynamic Motion and Communication in the Streptococcal C1 Phage Lysin, PlyC

The growing problem of antibiotic resistance underlies the critical need to develop new treatments to prevent and control resistant bacterial infection. Exogenous application of bacteriophage lysins results in rapid and specific destruction of Gram-positive bacteria and therefore lysins represent novel antibacterial agents. The PlyC phage lysin is the most potent lysin characterized to date and...

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Staphylococcal Virolysin, a Phage-induced Lysin

Virolysin is a lysin which appears in Staphylococcus aureus K(1) cells infected with phage P(14); together with phage, virolysin is released from phage-infected cells at the time of lysis. Autolysin is a lysin formed by uninfected cells of the K(1) strain; autolysin is released from uninfected cells by autolysis. They show the following similarities: Both agents act within the genus Micrococcus...

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ژورنال

عنوان ژورنال: Journal of General Microbiology

سال: 1963

ISSN: 0022-1287

DOI: 10.1099/00221287-32-1-29